Fig 1.

(a) Illustration of the MERS-CoV/EMC-2012 genome, the location of unique restriction sites are shown. (b) A full-length cDNA version of the MERS-CoV/EMC-2012 genome was assembled from three large fragments by homologous recombination. Primers sharing end homology are coded by color: black (vector), green (MF1), red (MF2) and blue (MF3). The full sequences of primers are shown in Table 1.0. (c) The complete MERS-CoV infectious clone system, including the pYES1L-BAC vector and regulator elements necessary for transcription of viral RNA (for interpretation of the references to color in this figure legend, the reader is referred to the online version of this article.)