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Supernatant from the BHK-21 and Vero cell overlay transfected with MERS-CoV/pYES1L plasmid was blind passaged three times to infect a fresh culture of Vero cells. These Vero cells were incubated for 3 days and observed for CPE. CPE was noted in the cells infected with the blind passage supernatant and not the negative control, indicating rescue of infectious virus (rMERS-CoV).
