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The replication kinetics of rMERS-CoV was compared with those of the clinical isolate MERS-CoV/EMC-2012 over a time course of 72 h. Vero cells were initially infected at an MOI of 0.001 and virus titre was measured every 24 h for 72 h. The viruses shared replication kinetics concluding that rMERS-CoV serves as a biological surrogate for the clinical isolate. Error bars represent the standard error of the mean +/− from the mean of three biological replicates.
