Table 1.

Oligo-nucleotide primers used for assembly of MERS-CoV cDNA by homologous recombination.

Primer Name	Use	Sequence (5′–3′)
CMVpF	MF1-F	CGCTGATACCGCCGCTATTAATAGTAATCAATTACGGGGTCAT
M-CoV6, 000R	MF1-R	ATTCTTATAAATAGGGTCATAAGTGTCAAACTCAGCCAACA
M-CoV6, 001F	MF2-F	GTTGGCTGAGTTTGACACTTATGACCCTATTTATAAGAATGGT
M-CoV18, 000R	MF2-R	GGCCAGTTACAATCTGAGACTGGAGCTTGTAATTAGTAAAAGA
M-CoV18, 001F	MF3-F	TCTTTTACTAATTACAAGCTCCAGTCTCAGATTGTAACTGGCC
BGTR	MF3-R	TTAACTGCGGCGAGGCAGCATATGGCATATGTTGCCAAACTCTAAACCAAATAC
pYES1L F	Vector	CATATGCCATATGCTGCCTCGCCGCAGTTAATTAAAGTCA
pYES1L R	Vector	TGATTACTATTAATAGCGGCGGTATCAGCG

Primers containing _∼ 30 bps of homology between MERS-CoV cDNA fragments (MF1, MF2 and MF3) and the pYES1L-BAC vector were used to amplify the regions in preparation for assembly by homologous recombination.