Table 5.

Limits of detection of each target used in this study are expressed as the minimum number of target gene copies in 25 μl PCR volume needed to produce a band visible by gel electrophoresis (detection limit of multiplex PCR) or to produce a reactivity of >2 PN ratio by electronic microarray (detection limit in electronic microarray).

Target pathogen	Target gene	Detection limit by multiplex PCR/RT-PCR	Detection limit by electronic microarray
BRD virus multiplex RT-PCR
BoHV-1	gB	100	1000
BRSV	G	1	1
BPI-3	M	1	10
BVDV 1	5’UTR	1	1
BVDV 2	5’UTR	1	10
BRD bacteria multiplex PCR
H. somni	16S rDNA	1	1
P. multocida	ulaR	1	1
M. bovis	16S rDNA	1	1
M. haemolytica	nmaA	10	10
	tbpB	10	100
	lktA	100	1000
BED virus multiplex RT-PCR
BCoV	N	1	1
BRoV A	VP6	1	1
BRoV C	VP6	100	1000
BRoV B	VP6	1	10
BToV (common)	N	1000	10000
BToV (Breda)	N	1000	1000
BVDV 1	5’UTR	1	1
BVDV 2	5’UTR	1	10
BED bacteria multiplex PCR
C. perfringens	plC	10	10
E. zuernii	ITS1	10	100
E. bovis	ITS1	10	10
C. parvum	COWP	100	1000
S. e. Dublin	A1118	10	100
S. e. Typhimurium	STM4493	100	100
E. coli	eaeA	10	10
	stx-1	10	100
	Lt	1	10
	K99	10	100