Table 1.
T cell reactive S protein peptides in monkeys vaccinated with VSVΔG-MERS.
| S peptidea |
T cell response to peptide |
|||
|---|---|---|---|---|
| Peptide # | Peptide sequence | Amino acid position | SFC/million PBMCb |
|
| i.m | i.n | |||
| S55 | VDLYGGNMFQFATLP | 271–285 | 1696 | 1720 |
| S124 | VFQNCTAVGVRQQRF | 616–630 | 1922 | 1860 |
| S154 | HPIQVDQLNSSYFKL | 766–780 | 1726 | 1620 |
| S174 | GGDFNLTLLEPVSIS | 866–880 | 1616 | 1660 |
| S221 | DKVNECVKAQSKRSG | 1101–1115 | 2306 | 2246 |
| S257 | IDLKELGNYTYYNKW | 1280–1295 | 1880 | 1842 |
a
Sequences were based on the S gene of MERS-CoV isolate Al-Hasa_4_2013.
b
The numbers of IFN-γ-positive T lymphocytes in PMBC samples expressed as spot-forming cells (SFC)/million PBMCs were determined by S overlapping peptides and IFN-ELISPOT assay.