Fig. 6.

2-DG inhibits PEDV replication and gene expression. (A) Vero cells were treated with 5 mM 2-DG for the indicated time. Cell lysates were analyzed by Western blot using antibody against GRP78. (B) Vero cells were infected with PEDV (MOI = 0.01) after pretreated with 2-DG 24 h for indicated concentrations. Supernatant (virions) and cell lysates were analyzed by Western blot using antibody against PEDV N. (C) 2-DG affected PEDV replication. The cells were incubated either in the presence or absence of 2-DG for 24 h before infected with PEDV (MOI = 1). The cells were harvested for the indicated time for RT-PCR analysis using primers specific for PEDV N gene. The ratio was the gene copy number of N gene in 2-DG treated and mock-treated cells. (D) Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. PEDV titers in the supernatants were measured by plaque formation assays. (E, F) 5 × 10⁵ Vero cells were infected with PEDV (MOI = 0.01) for 48 h after cells treated with various dosages of 2-DG for 24 h. Total RNA was extracted, and cDNA was synthesized by reverse transcription. The RT-PCR was used to analyze the viral RNA copy numbers in the cells and supernatants (virion).