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Sensitivity of nanoPCR assay (A) and the RT-PCR assay (B). A serial 10-fold dilution of PEDV RNA was used and analyzed by agarose gel electrophoresis. All experiments were repeated three times and similar results were obtained. M: DL2000; Lane 1: 2.7 ng/μL; Lane 2: 2.7 × 10−1 ng/μL; Lane 3: 2.7 × 10−2 ng/μL; Lane 4: 2.7 × 10−3 ng/μL; Lane 5: 2.7 × 10−4 ng/μL; Lane 6: 2.7 × 10−5 ng/μL; Lane 7: 2.7 × 10−6 ng/μL; Lane 8: 2.7 × 10−7 ng/μL.
