Table 2.
Performance of the multiplex PCR for clinical samples compared with the direct fluorescent antibody testing.
| Virus | No. of specimens by multiplex PCR VS. direct fluorescent antibody testing |
Accordance rate (%) | Kappa | PPA (%) | NPA (%) | ||||
|---|---|---|---|---|---|---|---|---|---|
| +/+ | +/- | -/+ | -/- | ||||||
| RSV | 14 | 1 | 4 | 333 | 98.58 | 0.841 | 77.78 | 99.70 | |
| PIV3 | 24 | 18 | 0 | 310 | 94.57 | 0.701 | 100 | 94.51 | |
| PIV1 | 2 | 6 | 0 | 344 | 98.30 | 0.394 | 100 | 98.29 | |
| PIV2 | 1 | 0 | 0 | 351 | 100 | 1.000 | 100 | 100 | |
| FluA | 2 | 2 | 0 | 348 | 99.43 | 0.664 | 100 | 99.43 | |
| FluB | 1 | 0 | 0 | 351 | 100 | 1.000 | 100 | 100 | |
PPA, positive percent agreement; NPA, negative percent agreement.