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. 2017 Dec 15;253:18–25. doi: 10.1016/j.jviromet.2017.12.005

Fig. 2.

Fig. 2

Detection of influenza A viruses subtypes using RT-LAMP and conventional one-step RT-PCR using HA gene primers. (a) agarose (2.5%) gel electrophoresis profile of RT-LAMP assays products (b) visual detection of green fluorescence in 0.2 ml PCR tubes using SYBR green dye under UV light. Lane M, 100 bp DNA marker; lane/tube 2, influenza H1N1; lane/tube 4, influenza H3N2; lane/tube 6, influenza pdm09/H1N1; lane/tube 1, 3, 5 are no template controls for H1N1, H3N2 and pdm09/H1N1 respectively. (c) agarose (2.5%) gel electrophoresis profile of conventional one-step RT-PCR reaction products. Lane M, 100 bp DNA marker; lane 1, no template control; lane 2, influenza A virus detection using M gene primers showing a band of 239 bp; lane 3, influenza A/pdm09/H1N1 showing a band of 261 bp; lane 4, influenza A seasonal H1N1 showing a band of 183 bp; lane 5, influenza A H3N2 showing a band of 360 bp. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)