Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 15;253:18–25. doi: 10.1016/j.jviromet.2017.12.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 3 — Sensitivity and specificity of RT-LAMP assay. (a) Sensitivity comparison of RT-LAMP assay (upper panel) and conventional RT-PCR (lower panel) for detection of IAVs using M gene primers, was performed using ten-fold serial dilutions of influenza A/California/7/2009(H1N1) strain with concentrations ranging from 100 to 0.0001 PFU/reaction tube. Detection limit of RT-LAMP assay was 0.01 PFU/reaction (upper panel) while conventional RT-PCR (lower panel) showed a detection limit of 0.1 PFU/reaction making RT-LAMP assay ten times more sensitive than conventional RT-PCR. Lane M, 100 bp DNA marker. (b) Influenza pdm09/H1N1 specific RT-LAMP reaction virus using HA gene primers was applied on other respiratory viruses to check the specificity of the reaction. Agarose electrophoresis profile of the RT-LAMP reaction products; lane M, 100 bp DNA maker; lane 1, no template control; lane 2, influenza A pdm09/H1N1; lane 3, influenza A/H1N1; lane 4, influenza A/H3N2; lane 5, influenza B virus; lane 6, respiratory syncytial virus (RSV); lane 7, human metapneumovirus (hMPV).