Table 1.
Details of primers used in RT-LAMP assay.
| Primer | Sequence (5′ → 3′) | Genome location | Primer length (bp) | Reference |
|---|---|---|---|---|
| Influenza A/M-gene | ||||
| Inf A-F3 | TGGTGCACTTGCCAGTTG | 375–392 | 18 | Poon et al., 2005 |
| Inf A-B3 | CCAGCCATCTGTTCCATAGC | 593−574 | 20 | |
| Inf A-FIP | TGCTGTGAATCAGCAATCTGTTACAGGA | F1c, 488-467 | 42 | |
| (F1c + F2) | TGGGAACAGTGACC | F2, 410–429 | ||
| Inf A-BIP | AGACAAATGGCTACTACCACCCGTAGT | B1c, 499–519 | 40 | |
| (B1c + B2) | GCTAGCCAGCACC | B2, 567-549 | ||
| A/pdm09H1/HA-gene (pandemic Influenza A) | ||||
| pdmH1-F3 | AGCTAAGAGAGCAATT | 170–185 | 16 | Nakauchi et al., 2011 |
| pdmH1-B3 | TTTCCCTTTATCATTAATGTAGGATTTG | 384−357 | 28 | |
| pdmH1-FIP | ACCTTTGTTCGAGTCATGATTTGGTTCTC | F1c, 264-244 | 51 | |
| (F1c + F2) | AGTGTCATCATTTGAAAGGTTT | F2, 189–213 | ||
| pdmH1-BIP | TAACGGCAGCATGTCCTCATTGTATGAA | B1c, 266–285 | 48 | |
| (B1c + B2) | TTTCCTTTTTTAACTAGCCA | B2, 345-319 | ||
| A/H1/HA-gene (seasonal Influenza A/H1) | ||||
| H1-F3 | CTGTTATGCACATTTACAGCT | 39–59 | 21 | This study |
| H1-B3 | TTCCTAAGATCCACCCGG | 258−241 | 18 | |
| H1-FIP | ACTGTGTCAACAGTGTCGGTTTTGCAGA | F1c, 124-105 | 44 | |
| (F1c + F2) | CACAATATGTATAGGC | F2, 65–86 | ||
| H1-BIP | ACACACTCTGTCAACCTGCTTTTCCAAT | B1c, 147–167 | 41 | |
| (B1c + B2) | TGTAGTGGGGCTA | B2, 225-208 | ||
| A/H3/HA-gene (seasonal Influenza A/H3) | ||||
| H3-F3 | ATAGTAAAACCGGGAGACAT | 754–773 | 20 | This study |
| H3-B3 | TGCTTAACATATCTGGGACA | 980−961 | 20 | |
| H3-FIP | TGGGTGCATCTGATCTCATTATTGATTTT | F1c, 868-844 | 48 | |
| (F1c + F2) | AGGGAATCTAATTGCTCCT | F2, 792–810 | ||
| H3-BIP | GCAAGTCTGAATGCATCACTCCTTTTTG | B1c, 878–899 | 48 | |
| (B1c + B2) | ATCCTGTTTACATTTTGGAA | B2, 949-928 | ||