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Limit of detection of RT-LAMP, conventional one-step RT-PCR and real-time RT-PCR assay using virus titre as plaque forming units (PFU).
| Influenza strain | Target gene | RT-LAMP (PFU/reaction) | Conventional RT-PCR (PFU/reaction) | WHO rRT-PCR (PFU/reaction) |
|---|---|---|---|---|
| A/California/7/2009 (H1N1) pdm09-like virus | M | 0.01 | 0.1 | 0.001 |
| A/California/7/2009 (H1N1) pdm09-like virus | HA | 0.01 | 1 | 0.01 |
| A/Texas/50/2012 (H3N2)-like virus | HA | 0.1 | 0.1 | 0.1 |
| A/Malaysia/2089302/2009 (H1N1)-like virus | HA | 0.1 | 1 | 0.01 |
