Table 4.
Identification of influenza A viruses and subtypes (H1N1, H3N2 and pdm09/H1N1) in clinical samples from Haryana, India, using RT-LAMP and conventional one-step RT-PCR, confirmed by WHO recommended rRT-PCR. Sensitivity and specificity were calculated by taking rRT-PCR as standard. The numbers in parentheses indicate the real-time RT-PCR results. ND: not determined.
| Detection and subtyping of influenza A viruses |
||||||
|---|---|---|---|---|---|---|
| Influenza A virus | Influenza A/H1N1 | Influenza A/H3N2 | Influenza A/pdm09/H1N1 | Negative | Total | |
| RT-LAMP assay | 30 (32) | 2 (2) | 7 (7) | 21 (23) | 137 (135) | 167 |
| Conventional RT-PCR | 27 (32) | 0 (2) | 7 (7) | 20 (23) | 140 (135) | 167 |
| Sensitivity/specificity of RT-LAMP assay (%) | 93.8/100 | ND | 100/100 | 91.3/100 | ||
| Sensitivity/specificity of conventional RT-PCR (%) | 84.4/100 | ND | 100/100 | 87/100 | ||