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. 2017 Sep 19;146:191–200. doi: 10.1016/j.antiviral.2017.09.010

Fig. 3.

Fig. 3

Celastrol induces antiviral IFN responses through HO-1 induction. (A) Celastrol induces IFN RNA levels upon HCV replication. Ava5 cells were treated with 0, 0.2, or 0.5 μM of celastrol for 3 days. RNA levels of IFN-α-5 and IFN-α-17 were measured by qRT-PCR. (B) HO-1 inhibitor antagonizes the inductive effect of celastrol on IFN expression levels. Ava5 cells were treated with 0.5 μM of celastrol and increasing concentrations of SnMP for 3 days. RNA levels of IFN-α-5 and IFN-α-17 were analyzed by qRT-PCR. (C) Celastrol induces IFN secretion upon HCV replication. Ava5 cells were treated with 0, 0.2, or 0.5 μM of celastrol for 3 days. The supernatant was then collected to analyze the secretion level of IFN-α by ELISA. Error bars denote the mean ± SD of three independent experiments. *P < 0.05.