Table 1.
Inter and intra-assay variation of the colorimetric PEDV virus neutralization assay*.
| Replicate 1# |
Replicate 2# |
|||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| Negative | ||||||
| Visual | 1:2 | >1:2 | >1:2 | >1:2 | >1:2 | >1:2 |
| Colorimetric* | 1:2 | >1:2 | >1:2 | >1:2 | >1:2 | >1:2 |
| Positive | ||||||
| Visual | <1:1024 | <1:1024 | <1:1024 | 1:256 | 1:512 | <1:1024 |
| Colorimetric | <1:1024 | <1:1024 | <1:1024 | 1:256 | 1:512 | <1:1024 |
A cut-off value of ≤ 0.53 OD was used to distinguish between positive and negative wells for the colorimetric assay.
Two independent assays with triplicate values each.