Fig. 1.
Ribavirin inhibits ZIKV replication in Vero cells. (A–D) Vero cells were infected with ZIKV at an MOI of 5 × 10−5. After 1 h adsorption, 2% complete MEM containing both ribavirin and IFN-β (A, D), ribavirin (B) or IFN-β (C) was added on the cells. Cells were incubated for 4 days, total RNA was isolated from Vero cells, and expression of ZIKV RNA was analysed by quantitative RT-PCR assay. The relative amount of viral RNA expression normalized against the corresponding level of β-actin mRNA is shown. ‘ZIKV -’ represents uninfected cells. Data represent mean values ± S.D. of three independent experiments. **, P < 0.01. *, P < 0.05.