Fig. 4.

Analysis of ZIKV-induced apoptosis and cell death by confocal microscopy and flow cytometry. (A, B) Vero cells were infected with ZIKV at MOI of 5 × 10⁻³. Following 1 h of adsorption at 37 °C, cells were cultured with 2% MEM containing ribavirin (15.6–2000 μg/mL). Four days post-infection, cells were observed by microscopy (A), or stained with annexin V and Zombie Red and analysed by flow cytometry (B). ‘ZIKV -’ represents uninfected cells. Data are representative of two independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)