Table 2.
Stability of HSV-1 G-quadruplex folding sequences measured by UV and CD, and in the presence/absence of K+.
| Sequence | Tm (UV 100 mM K+) | Tm (CD 100 mM K+) | Tm (CD no K+) | ΔTm[100 mM/no K+] (CD) | Tm (CD 100 mM K+, BRACO-19 16 μM) |
|---|---|---|---|---|---|
| gp054a | 87.4 ± 0.4 | 88.1 ± 1.3 | 48.0 ± 1.1 | 40.1 | >95 |
| gp054b | 88.0 ± 0.4 | 87.5 ± 1.1 | 48.4 ± 1.6 | 39.1 | >95 |
| gp054c | 92.3 ± 0.8 | 90.8 ± 0.3 | 51.7 ± 1.3 | 39.1 | >95 |
| gp054d | 91.5 ± 0.1 | 90.7 ± 0.3 | 71.3 ± 2.6 | 19.4 | >95 |
| gp054e | 91.7 ± 0.6 | 87.5 ± 0.5 | 58.0 ± 0.8 | 29.5 | >95 |
| gp054f | 88.1 ± 0.1 | 88.0 ± 0.2 | 51.9 ± 1.4 | 36.1 | >95 |
| un1 | nd | nd | 40.8 ± 9.8 | nd | >95 |
| un2 | >95 | >95 | 50.3 ± 0.9 | >44.7 | >95 |
| un3 | >95 | >95 | 34.7 ± 4.7 | >60.3 | >95 |