Fig. 2.

Characterization of mAbs m27f and 21C11. (A) Western blot analysis. Vero cell monolayers were infected with HSV-1 or HSV-2 at an MOI of 5 pfu per cell. At 48 h p.i., cells were harvested for Western blot analysis using m27f or 21C11 as the primary antibody. β-actin antibodies were used as control. (B) IFA. Cells were infected with HSV-1 or HSV-2 at an MOI of 5 pfu per cell. At 48 h p.i., cells were fixed and subjected to IFA using m27f or 21C11 as the primary antibody and FITC-conjugated goat anti-mouse IgG as the secondary antibody. (original magnification, ×100) (C) and (D) Virus neutralization assay. HSV-1 or HSV-2 (100 pfu/well) was incubated with indicated serially diluted antibodies m27f, 21C11 or pre-immune serum. At 72 h p.i., cells were stained and the number of plaques counted. The percentage inhibition was determined relative to the negative control. Representative data (mean ± SEM) from three independent experiments are shown.