Fig. 3.

Detailed epitope mapping of m27f. (A) Schematic diagram of the gD protein and accurate truncation strategy. The structure of the full-length gD protein is shown at the top; the truncated gD proteins used for the sequential mapping experiment are shown below. Numbers indicate amino acid positions. The domain (pro-fusion) was defined according to Fusco et al. (2005). (B) Epitope mapping of m27f. The truncated gD proteins expressed by pMAL-C2x vector were presented as antigens. MAb m27f was used as the primary antibody for Western blot analysis. (C) Alignment analysis. Residues 292 to 297 are highly conserved in both HSV-1 and HSV-2. Sequence alignment was performed with DNAMAN V6.