Fig. 6.

m27f inhibits syncytium formation of HSV-1 and HSV-2. (A) Vero cells were infected with HSV-1 or HSV-2 at an MOI of 5 pfu per cell and then incubated in DMEM containing 2% FBS in the presence of antibodies, m27f (500 μg/ml) and 21C11 (500 μg/ml). Mouse IgG (500 μg/ml), or medium alone were used as control. After 48 h, cells were fixed and then stained with HE staining. The inhibition of syncytium formation was observed by light microscopy. Bar represents 100 μm. The experiments were repeated at least twice. (B) Syncytium formation of Vero cells with different treatment as shown in panel A was quantified by counting the number of nuclei in syncytia of randomly chosen area. The percentage inhibition was determined relative to no antibody treatment group. Only syncytia containing 3 or more nuclei were analyzed. Data were representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.