Table 5.

Test parameters for the BCV multiplex and BCV ELISA: Sensitivity, specificity, and estimates of true prevalence (TP) in the two sub-populations. Cut-off alternative 1- 9 represents different cut-off alternatives for the BCV multiplex (presented in Table 2). The BCV ELISA cut-off was fixed at sample positive >10 PP for all alternatives.

	Test								Sub-population
	BCV multiplex				BCV ELISA				Pop 1		Pop 2
Parameter	Se		Sp		Se		Sp		TP		TP
Cut-off alternative	Median	[95% PCI]	Median	[95% PCI]	Median	[95% PCI]	Median	[95% PCI]	Median	[95% PCI]	Median	[95% PCI]
1	99.9	[99.4;100]	77.3	[69.8;84.8]	99.0	[96.9;100]	99.5	[97.1;100]	94.0	[91.0;96.3]	61.5	[56.2;66.7]
2	99.6	[98.6;100]	91.1	[85.4;96.0]	99.4	[97.8;100]	97.4	[93.4;99.7]	93.5	[90.5;95.8]	60.3	[55.1;65.5]
3	99.5	[98.1;100]	93.1	[88.0;97.2]	99.5	[98.1;100]	92.3	[87.3;96.7]	93.1	[90.1;95.5]	58.0	[52.7;63.2]
4	98.9	[96.8;100]	94.3	[89.5;98.1]	99.5	[98.0;100]	87.5	[81.1;93.3]	92.6	[89.4;95.2]	55.8	[50.3;61.2]
5	99.0	[96.8;100]	94.3	[89.5;98.1]	99.5	[98.0;100]	81.6	[75.0;87.8]	92.2	[88.9;94.9]	52.4	[47.0;58.0]
6	97.9	[95.1;99.7]	94.3	[89.5;98.1]	99.5	[98.0;100]	79.8	[72.6;86.8]	91.8	[88.2;94.8]	51.5	[45.9;57.3]
7	90.6	[86.6;94.2]	94.3	[89.5;98.1]	99.4	[97.9;100]	82.6	[73.5;92.3]	92.1	[88.3;95.0]	53.3	[46.8;60.1]
8a	99.9	[99.3;100]	93.7	[88.8;97.8]	99.5	[98.1;100]	99.6	[97.6;100]	93.5	[90.6;95.7]	61.2	[56.0;66.2]
9a	97.1	[94.0;99.4]	99.2	[96.9;100]	99.8	[99.1;100]	80.4	[73.1;87.5]	91.5	[87.8;94.5]	51.6	[46.1;57.1]

^aOnly BCV-A antigen included.