Fig. 7.

Intracellular localization of EGFP fusion proteins containing segments of N protein deletion mutants. Vero cells were transfected with pEGFP-N1 vector only or with different deletion mutants fused to N-terminal of EGFP. After 24 h cells were analyzed by direct fluorescence using confocal microscopy. The panels represent the distribution of fluorescence signals in the cells transfected with EGFP, EGFP-N and deletion mutants as indicated. ((C), (F), (I), (L), (N), (Q) and (T)) are phase-contrast of the fluorescence images ((B), (E), (H), (K), (M), (P) and (S)) of the same cells, respectively. Arrow points to the cell nucleus or to the nucleolus.