Fig. 5.

Inhibition of calicivirus particle formation in CRFK-monolayer after infection with the FCV/Dresden/2006/GE strain (GenBank accession No. DQ424892). Two siRNA (siR19 and siR20) targeting conserved sequences in the 5′-UTR and 3′-UTR, respectively, were used. The siRNA (330 nM) was transfected into the CRFK cells 5 h prior to infection with FCV. Plaque assays were performed as described by others (Gray, 1999), yielding a viral titre of 500 PFU/ml. The siRNA completely inhibited viral multiplication in the CRFK cells.