Fig. 6.
Inhibition of the activity of norovirus and sapovirus RNA-dependent RNA polymerases by nucleoside analogues. (A) Assessment of the activity of the norovirus RNA-dependent RNA polymerase was determined as previously described (Rohayem et al., 2006a, Rohayem et al., 2006b). (B) Assessment of the activity of the sapovirus RNA-dependent RNA polymerase NS7pol was determined as previously described (Fullerton et al., 2007). 2′-Fluor-UTP, 2′-fluorouridine-5′-triphosphate. 2′-O-Methyl-UTP, 2′-O-methyluridine-5′-triphosphate. 2′Ara-UTP, 2′-arauridine-5′-triphosphate; 3′-deoxy-UTP; 3′-deoxyuridine-5′-triphosphate. 2′-O-Methyl-GTP, 2′-O,methylguanidine-5′-triphosphate. The mean and the standard error of the mean of three independent experiments are shown. CPM, counts per minute. Each of the compounds was incubated at a concentration of 50 μM.