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. 2007 Oct 25;131(2):293–298. doi: 10.1016/j.virusres.2007.09.009

Table 1.

Kinetic parameters of the degradation of selected fluorogenic substrates by CHIKV nsP2 protease

Substrate Junction Km (μM) kcat (×10−6 s−1) kcat/Km (s−1 M−1)
Boc-AGA-MCA nsP1/nsP2 2,422 932 0.38
Boc-AGG-MCA nsP3/nsP4 232 374 1.61
Dabcyl-AGAGIIE-Edans nsP1/nsP2 ND ND ND
Dabcyl-AGGYIFSE-Edans nsP3/nsP4 61 140 2.3

Non-prime substrate sequences are indicated in bold and prime substrate sequences are shown in italics. Protease activity was assayed in Tris 50 mM pH 9.5, NaCl 10 mM using a substrate concentration ranging from 25 to 200 μM. Standard reaction contained the CHIKV nsP2pro protease at a concentration of 0.5 μM. The kinetic parameters for the degradation of Dabcyl-AGAGIIE-Edans have not been determined (ND) because of the too low activity of the protease towards this substrate.