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. 2006 Dec 6;74(2):142–149. doi: 10.1016/j.antiviral.2006.11.004

Fig. 2.

Fig. 2

Quantitative analysis of luciferase activity in cells transfected with siRNA expressing plasmids: (A) HEK293T cells were co-transfected with pLucF–gp120 and pSliencer-2.1-U6-siRNA plasmids (HIVsiRNA1, HIVsiRNA2, HIVsiRNA3, HIVsiRNA4). pSliencer-2.1-U6 plasmid was used as a control; (B) Bel-7402 cells were co-transfected with pLucF–HBs plasmid and pSliencer-2.1-U6-siRNA (HBSsiRNA2, HBV–HIVsiRNA, HIVsiRNA3) plasmids. pSliencer-2.1-U6 vector was used as a control; (C) HEK293T cells were co-transfected with pLucF–gp120 and pSliencer-2.1-U6-siRNA (HBSsiRNA2, HBV–HIVsiRNA, HIVsiRNA3) plasmids. pSliencer-2.1-U6 was used as vector control. About 48 h after transfection, cells were lysed and luciferase activities were determined by luminometer.