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. 2006 Dec 6;74(2):142–149. doi: 10.1016/j.antiviral.2006.11.004

Fig. 5.

Fig. 5

Levels of RNA expression determined by semi-quantitated RT-PCR analysis. Total RNA was used as template to synthesize cDNA. HIVgp120-specific primers were then applied for detection in (A) and (C), and HBs specific primers in (D). (A) HEK293T cells were co-transfected with pCMV–HIVgp120 and HIVsiRNA1 (lane 1), HIVsiRNA2 (lane 2), HIVsiRNA3 (lane 3), HIVsiRNA4 (lane 4), and pSliencer-2.1-U6 (lane 5, as a vector control). Lanes 6–10 were β-actin controls. (B) HEK293T cells were co-transfected with pCMV–HIVgp120 and HIVsiRNA3 (lane 1), HBV–HIVsiRNA (lane 2) or pSliencer-2.1-U6 (lane3, as a vector control). As control, HEK293T cells were also transfected with pCMV–HIVgp120 only (lane 4). Lanes 5–8 were β-actin controls. (C) HEK293T cells were co-transfected with pNL4-3.luc.R-E- and HBSsiRNA2 (lane 1), HBV–HIVsiRNA (lane 2), HIVsiRNA3 (lane 3), or pSliencer-2.1-U6 (lane 4, as a vector control). As control, HEK293T cells were also transfected with pNL4-3.luc.R-E- only (lane 5). Lanes 6–10 were β-actin controls. (D) HepG2.2.1.5 cells were transfected with HBSsiRNA2 (lane 1), HBV–HIVsiRNA (lane 2), HIVsiRNA3 (lane 3) or pSliencer-2.1-U6 (lane 4, as a vector control). Lanes 5–8 were β-actin controls. Lane M indicates DNA markers.