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. 2006 Dec 6;74(2):142–149. doi: 10.1016/j.antiviral.2006.11.004

Fig. 7.

Fig. 7

Dual siRNA generation system simultaneously inhibited the replication of HIV-1 and the expression of HBV. COS-7 cells were co-transfected with plasmid pNL4-3, pCMV–HBs and siRNA (HBSsiRNA2, HBV–HIVsiRNA, and HIVsiRNA3) or empty vector as control. The level of p24 protein in culture media from transfected cells was then determined by enzyme-linked immunosorbent assay kit (A) and HBsAg concentrations in the transfected cells were measured by ELISA using HBsAg diagnostic kit at 2 days after transfection (B).