Table 1.
Primers and probes information for real-time PCR target genes.
| Panel | Primers/probes | Sequence (5′- 3′) | Amplicon size (bp) | Accession number |
|---|---|---|---|---|
| Panel 1 | Mora bovis-F | GGTGACGACCGCTTGTTT | 63 | EF436233.1 |
| Mora bovis-R | ATCATCGCCTTCATCTCCAG | This study | ||
| Mora bovis-Pr | 5′- Texas Red- CGATCGTTGCCTTTACCACC -3′ | |||
| Mora bovo-F | GGTGATATTTATCATGAAGTTGTGAAA | 88 | KP410780.1 | |
| Mora bovo-R | TYTCAATTCATAATCACGATACTCAAG | This study | ||
| Mora bovo-Pr | 5′-VIC-CCAAGATACTGCGGTAGGTAAACG-3′ | |||
| 18S-F | GGAGTATGGTTGCAAAGCTGA | 100 | DQ222453.1 | |
| 18S-R | GGTGAGGTTTCCCGTGTTG | (Bai et al., 2018) | ||
| 18S-Pr | 5′-Cy5-AAGGAATTGACGGAAGGGCA-BHQ2–3′ | |||
| Panel 2 | Myco bovis-F | GCTGATGGCGGTATACAACA | 110 | KX772802.1 |
| Myco bovis-R | GCTTTGGTTTTGTGAAACTC | This study | ||
| Myco bovis-Pr | 5′- MAX-CGCTTAAAACGCTTAATATAAACATCC-3′ | |||
| Myco bovo-F | AGCTATGGCAGGGGACAAC | 66 | JQ390390 | |
| Myco bovo-R | CCACGTTCAATGTCTTTACGG | This study | ||
| Myco bovo-Pr | 5′-FAM- TGGTGTTCTACTCCGTGG-3′ | |||
| BHV-1-F | TCGCGCACTGAGCAGAC | 86 | KR230748.1 | |
| BHV-1-R | CCGTCGGTTGTAGAAAGCAG | This study | ||
| BHV-1-Pr | 5′- Texas Red-CCCGGGCTGGTAAACCTG-3′ |
Note: F: Forward primer; R: Reverse primer; Pr: Probe; FAM: Fluorescein, or Fluorescein amidite; Texas Red: sulforhodamine 101 acid chloride; MAX: Rhodamine (HEX or VIC equivalent); VIC: 2′-chloro-7'phenyl-1,4-dichloro-6-carboxy-fluorescein; Cy5: Cyanine 5; BHQ: Black-Hole Quencher. All sequence accessions listed in the table are submitted by other researchers. They are listed as a representative accession from which the primers and probes were designed.