Table 2.
Analytical comparison of PCR amplification efficiencies, correlation coefficients and limit of detection between singleplex and multiplex reactions.
| Target | PCR reaction format | PCR amplification efficiency (%) | Correlation coefficient (R2) | Limit of detection (copy/reaction) |
|---|---|---|---|---|
| Moraxella bovis | singleplex | 105.9 | 0.993 | 20 |
| multiplex | 101.1 | 0.992 | 19 | |
| Moraxella bovoculi | singleplex | 99.4 | 0.998 | 21 |
| multiplex | 92.8 | 0.992 | 23 | |
| Mycoplasma bovis | singleplex | 98.1 | 0.998 | 22 |
| multiplex | 99.1 | 0.996 | 25 | |
| Mycoplasma bovoculi | singleplex | 99.2 | 0.998 | 23 |
| multiplex | 96.6 | 0.996 | 24 | |
| Bovine herpes virus 1 | singleplex | 97.0 | 0.999 | 26 |
| multiplex | 92.3 | 0.998 | 26 |
Note: PCR amplification efficiency (E) is calculated by E = 10(−1/slope)−1.