Table 7.

Genes of the Pathway Set with expression significantly up-regulated by GSK983 in W12 or HKC cells.

Gene symbol	Cell type	Untreated abundancea		Fold increase due to GSK983
		log(2) at zero time	S.E.	10 nM, 24 h	10 nM, 48 h	100 nM, 24 h	100 nM, 48 h
CEL	W12	−22.4	0.2	–	14.1	3.0	10.6
COL10A1	W12	−15.0	0.4	0.8b	2.0	3.6	2.4
CPT1B	W12	−13.9	0.1	2.2	2.2	3.2	6.4
CSF2	W12	−13.5	0.3	1.5b	1.7	3.7	4.9
DCN	W12	−11.0	0.1	1.5b	2.1	2.3	15.4
DMPK	W12	−12.6	0.1	0.9b	2.1	2.9	4.1
ERN2	W12	−17.0	0.4	0.7b	2.6	1.7b	2.8
FMOD	W12	−17.1	0.3	1.0b	1.5	1.7	2.5
IFIT1c	W12	−12.0	0.1	2.6	2.0	5.5	5.5
IL6c	W12	−14.0	0.4	2.5b	1.8	3.8	10.2
IL8	W12	−9.3	0.2	1.8b	1.4	2.1	2.3
ISG15c	W12	−9.9	0.1	2.5	3.0	4.3	9.3
LIFR	W12	−14.6	0.2	2.1b	1.5	1.3b	2.2
LRP1	W12	−12.6	0.3	1.2b	3.5	3.7	3.5
NR1H3	W12	−12.1	0.2	0.6b	1.8	6.8	2.7
OAS1c	W12	−11.0	0.3	1.1b	1.7	2.2	5.0
OAS2c	W12	−10.3	0.4	2.1	1.8	1.9	2.9
OASLc	W12	−10.6	0.2	1.8	2.2	6.7	10.9
CCL5	W12	−16.4	0.4	7.9b	41.5	3.1	47.4
SNAP25	W12	−18.3	1.0	1.2b	3.7	1.7b	4.7
TLR5	W12	−15.7	0.4	1.9b	1.7	2.4	3.1
TNFSF10c	W12	−14.5	0.1	3.7	1.8	4.3	8.4
ADAMTS5	HKC	−16.3	0.3	2.6	2.3	1.3b	7.5
ABCD1	HKC	−15.1	0.7	2.0	4.5	2.3	3.7
ABCG1	HKC	−11.3	0.4	2.5	3.1	5.0	4.8
BIK	HKC	−9.1	0.1	2.2	1.8	2.3	3.7
CDKN1A	HKC	−4.7	0.1	1.7b	1.6	1.8	4.3
CSF2	HKC	−11.6	0.1	1b	4.1	2.8	5.8
MDM2	HKC	−6.5	0.1	2.1	3.2	2.4	2.9
MGP	HKC	−13.2	0.1	1.9	2.9	2.0	3.0
MMP1	HKC	−9.5	0.2	5.8b	2.2	5.2b	5.0

Growing cultures of W12 and HKC cells were treated with 0, 10 and 100 nM GSK983 and 100 nM GSK984. RNA was prepared from samples taken at 0, 24 and 48 h after addition of the compound and mRNA levels of each of the 546 genes of the Pathway Set were measured in quadruplicate by RT-qPCR. Changes in expression levels due to GSK983 treatment were calculated as described in Section 2.

^aRelative to GADPH mRNA (Ct ∼ 15).

^bFold change not significantly different from 1.

^cInterferon-stimulated gene (ISG).