Table 8.

Effect of GSK983 in W12 and HKC cells on expression of other interferon-stimulated genes not in the Pathway Set.

Gene symbol	Cell type	Untreated abundancea		Fold increase due to GSK983
		log(2) at zero time	S.E.	10 nM, 24 h	10 nM, 48 h	100 nM, 24 h	100 nM, 48 h
IFI6	W12	−8.8	0.12	1.1b	2.2	2.3	7.3
IFI16	W12	−9.9	0.10	5.2	1.6	1.3	1.2b
IFI44	W12	−10.7	0.15	2.9	2.7	2.9	4.0
IRF3	W12	−9.5	0.14	7.8	1.3	1.5	1.2b
IRF9	W12	−10.2	0.15	1.0b	1.7	1.6	4.5
IFI6	HKC	−2.3	0.08	1.3b	1.4b	1.1b	1.4b
IFI16	HKC	−11.5	0.25	2.3b	2.7	0.6b	0.8b
IFI44	HKC	−7.8	0.11	1.9b	1.7b	0.7b	0.5b
IRF3	HKC	−11.1	0.29	3.7	2.4	1.1b	0.8b
IRF9	HKC	−5.3	0.12	1. 3b	1.5b	1.6	1.5b

The RNA samples from the experiment of Table 7 were analyzed in quadruplicate by RT-qPCR for mRNA levels of each of the 5 genes listed above. Changes in expression levels due to GSK983 treatment were calculated as described in Section 2.

^aRelative to GADPH mRNA (Ct ∼ 15).

^bFold change not significantly different from 1.