Figure 3.

Effect of LPS E. coli 026:B6 and LPS E. coli K-235 on the antimicrobial activity of HBD2 and HBD3 (A) E. coli (B) S. aureus. Ratios (w/w 1:1 and w/w 1:5) are indicated in parentheses. Bacteria were incubated with the peptides at their MBC in 10 mM sodium phosphate buffer (pH 7.4) containing LPS for 2 h at 37 °C and suitably diluted aliquots were plated on LB agar plates, which were incubated at 37 °C for 18 h. Colonies formed were counted and percentage bacteria killed was determined.