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. 2006 Sep 11;122(1):20–27. doi: 10.1016/j.virusres.2006.06.005

Fig. 3.

Fig. 3

Cellular localization of HA-3b and HA-3bΔ124-154 proteins. Subcellular fractionation was performed to separate the cells into cytoplasmic (C), membrane (M) and nuclear (N) fractions. Western analyses were performed using with anti-HA (upper panel) and anti-actin (lower panel) monoclonal antibodies for each protein: (A) HA-3b; (B) HA-3bΔ124-154. The migration of protein molecular weight markers is indicated on the left. (C) The cellular localization of HA-3b, HA-3bΔ124-154, HA-Bax were analysed by indirect immunofluorescence at 18 h post-transfection. Mock transfected cells showed the specificity of the anti-HA monoclonal antibody.