Table 2.
Amino acid changes in the spike protein of CRCoV
| Amino acid | T101 | T4182 | Isolate 4182 p13 |
|---|---|---|---|
| 36 | I | V | V |
| 74* | T | T | I |
| 166* | D | D | N |
| 171* | M | M | I |
| 175 | N | S | S |
| 192 | P | S | S |
| 210 | S | F | F |
| 267* | F | F | S |
| 501 | F | S | S |
| 692 | G | A | A |
| 757 | W | C | C |
| 758 | G | V | V |
| 769 | T | S | S |
| 786 | P | S | S |
| 792 | H | Q | Q |
| 827 | P | S | S |
| 974 | A | V | A |
| 977 | F | S | S |
| 983* | P | P | R |
| 985* | S | S | P |
| 1018* | S | S | A |
| 1063 | K | R | R |
| 1300* | Y | Y | F |
| 1360 | S | L | L |
The amino acid sequence of the complete spike ORF of CRCoV isolate 4182 was compared to the spike amino acid sequence obtained from CRCoV-RNA extracted from tracheal samples T101 and T4182. Amino acid positions that acquired changes during culture of CRCoV isolate 4182 in HRT-18 cells are indicated by an asterisk.