Fig. 3.

HSV-2 infection promotes Erk MAPK activation, HCWE treatment showed no effect on Erk activation. HeLa 229 cells were infected with HSV-2 (MOI = 5) in the presence or absence of 450 μg/ml HCWE. Cell lysates were harvested at 3 and 6 h PI, and the levels of phosphorylated forms of Erk1/2, p38 and JNK1/2 as well as total Erk2, p38 and JNK1/2 proteins were determined by immunoblotting analysis. Anisomycin (Aniso) at 5 μM was used as a positive control since it activates all three MAPK classes examined.