Table 3.
Validation of gene regulation by real-time RT-PCR
| Gene name | Fold change in expression at PI time points |
|||||||
|---|---|---|---|---|---|---|---|---|
| 6 h |
24 h |
48 h |
72 h |
|||||
| Array | qRT | Array | qRT | Array | qRT | Array | qRT | |
| Transcriptional repressor protein yy1 | 1.28 | 0.87 | 6.84 | 2.14 | 1.26 | 1.62 | 1.24 | 1.86 |
| Signal transducer and activator of transcription 1-alpha/beta | 3.03 | 2.54 | 1.45 | 0.53 | 2.80 | 2.82 | 0.96 | 0.31 |
| Elongation factor 1-alpha 1 (ef-1-alpha-1) | 3.04 | 1.74 | 1.34 | 1.23 | 3.05 | 2.63 | 0.94 | |
| AAH01874 similar to sequestosome 1 | 1.09 | 2.07 | 1.52 | 2.73 | 1.08 | 1.86 | 0.95 | 0.00 |
| Cd80-like protein precursor | 0.74 | 0.90 | 2.77 | 4.43 | 0.96 | 0.32 | 0.97 | 0.90 |
| Ornithokinin receptor | 0.69 | 0.40 | 1.05 | 0.65 | 0.99 | 0.70 | 1.21 | 1 |
| Interferon-induced protein with tetratricopeptide repeat | 21.9 | 1.8 | 35.2 | 57.6 | 13.9 | 2.5 | 0.99 | 3.2 |
| Embryo fibroblast protein 1 precursor | 2.14 | 3.63 | 1.11 | 1.51 | 1.20 | 2.46 | 0.46 | 0.19 |
| Lumican precursor (lum) (keratan sulfate proteoglycan | 1.10 | 1.10 | 1.14 | 1.31 | 0.85 | 2.29 | 1.11 | 2.14 |
| Complement c3f [fragment] | 0.87 | 2.22 | 1.29 | 1.46 | 1.13 | 2.22 | 0.99 | 3.03 |
| Interferon-induced protein 6–16 precursor | 0.85 | 3.86 | 21.8 | 1.16 | 5.66 | 1.12 | 2.22 | |
| Thrombospondin 2 precursor. | 1.12 | 0.87 | 0.84 | 0.51 | 0.85 | 5.85 | 0.79 | 11.3 |
| Tata element modulatory factor | 0.959 | 0.93 | 0.927 | 1.27 | 0.930 | 8 | 0.963 | 0.34 |
| Alpha-interferon inducible protein (fragment) p27-h | 0.86 | 1.4 | 1.86 | 19.02 | 1.481 | 12.9 | 1.47 | 1.63 |