Fig. 3.

Effects of flavonoids upon HCMV DNA levels (a) and reversibility of flavonoids (b). (a) HCMV DNA replication was determined by dot (Southern) blotting hybridization at 72 h post infection. Controls were performed for uninfected cells, HCMV-infected cells, and HCMV-infected cells treated with 50 μM ganciclovir (GCV). BAI indicates treatment with 20 μM baicalein and GEN indicates treatment with 50 μM genistein. Fractions indicate the dilution factor of samples applied to membranes. This experiment was performed independently four times with substantially the same results. (b) Reversibility of flavonoids. HCMV DNA replication was blocked for 72 h with 20 μM baicalein (BAI) or 50 μM genistein (GEN) then released from drug pressure and allowed to replicate for an additional 72 h. Viral DNA levels were determined at 24 h intervals by dot blotting. This experiment was reproduced twice.