Table 2.
Primers used for amplification of the complete genome of PDCoV.
| Primers | Sequence (5’→3’) | Nucleotide position* | Fragment size (bp) |
|---|---|---|---|
| PDC-1-F PDC-3300-R |
ACATGGGGACTAAAGATAAAAATTATAGC GCTCATCGCCTACATCAGTG |
1-29 3281-3300 |
3300 |
| PDC-3091-F PDC-4860-R |
CGGATTTAAAACCACAGACT ACGACTTTACGAGGATGAAT |
3091-3110 4841-4860 |
1770 |
| PDC-4741-F PDC-6420-R |
CTCCTGTACAGGCCTTACAA TCACACGTATAGCCTGCTGA |
4741-4760 6401-6420 |
1680 |
| PDC-6291-F PDC-8041-R |
CTCAATGCAGAAGACCAGTC CAGCTTGGTCTTAAGACTCT |
6291-6310 8041-8060 |
1751 |
| PDC-7920-F PDC-9660-R |
GGTACTGCTTCTGATAAGGAT TAGGTACAGTTGTGAACCGA |
7920-7940 9641-9660 |
1741 |
| PDC-9463-F PDC-10579-R |
TACTCTTCACAGCCTTCAC TTCTTCACAATAGTGTAGCG |
9463-9482 105601-10579 |
1117 |
| PDC-10560-F PDC-12847-R |
CGCTACACTATTGTGAAGAA TTCGTAGGGCTCAAGATA |
10560-10579 12829-12847 |
2288 |
| PDC-12301-F PDC-14952-R |
TCCAGATGACCGTTGCGTAT CCAACAGAGTCGGGTAAA |
12301-12320 14935-14952 |
2652 |
| PDC-14703-F PDC-17185-R |
CACCCATAACGAAGAACC CCGATGAGTGTCGTAGCG |
14703-14720 17168-17185 |
2483 |
| PDC-17165-F PDC-19203-R |
TGCCGCTACGACACTCAT TCCGCTAAAGGAGAATAGGTTG |
17165-17182 19182-19203 |
2039 |
| PDC-18485-F PDC-21079-R |
TGCTACCCAATCTTACAGT GCAAATACTCCGTCTGAAC |
18485-18503 21061-21079 |
2594 |
| PDC-20761-F PDC-23825-R |
GTCTTACCGTGTGAAACCCC AACCACGAGACTGTAAGCAA |
20761-20780 23806-23825 |
3064 |
| PDC-23804-F Oligo(dT) |
TTTTGCTTACAGTCTCGTGGTT TTTTTTTTTTTTTTTTTT |
23804-23825 25432 |
1629 |
*
Nucleotide position of each primer was numbered based on the HKU15-155 strain (GenBank accession no. JQ065043).