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The APC concept. (A) The APC insert is made by custom synthesized tandems of forward and reverse complement priming sequences ligated into the multiple cloning site of pUC57 to make a circular and clonable APC construct. Each PCR product amplified from the APC construct has a unique identifiable sequence useful for further PCR product verification and quality control. (B) An APC construct carrying multiple custom synthetic APC inserts. Not to scale.
