Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 4;77(2):95–107. doi: 10.1016/j.antiviral.2007.09.002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 8 — PPMO-mediated suppression of PRRSV replication in porcine alveolar macrophages. (A) Protection of PAM from PRRSV CPE development. Infection of PAM with VR2385 led to severe CPE development (see +VR2385, −PPMO) in comparison with uninfected control cells (−VR2385, −PPMO). Application of PPMO 5UP2, 5HP, or a combination of 6P1 + 7P1 at 16 μM resulted in inhibition of CPE development, while CP1 did not. (B) Reduced PRRSV infection in PAM detected by IFA with SDOW17 antibody. Specific fluorescence is clearly visible at 12 h after VR2385 inoculation. Treatment with PPMO 5UP2, 5HP, 6P1 + 7P1 resulted in reduction of virus replication, while CP1 did not. The images below the green fluorescence images were taken with a DAPI filter to show the total number of living cells. (C) Titration of virus yield from PAM cells after PRRSV infection and PPMO treatment. Virus titers are shown as TCID₅₀ (log₁₀/ml). “Mock” sample is virus inoculation with no PPMO treatment. Statistical significance of the difference in viral yield compared to mock-treatment ^*P < 0.05. The experiment was repeated three times and error bars are shown.