Fig. 4.

Inhibition of VACV and CPXV growth by SP600125 is independent of JNK1/2. WT (A) and JNK1/2 KO (B) cells were either left untreated or treated with SP600125 (40 μM), or with JNK Inhibitor VIII (4 μM) prior to VACV or CPXV infection at an MOI of 10. At 24 h.p.i, total cell lysates were assayed for viral production. (C) Cells were left uninfected (MOCK) or were treated with SP600125 or JNKi at (4 μM) (lanes 5, 6 and 8, 9), as indicated. Total cell lysates (40 μg) were prepared, subjected to western blot and probed with anti-phospho c-JUN (Ser 73) – upper panels, or probed with anti-β tubulin antibody (1:3000) as a loading control – lower panels. Data are representative of three distinct experiments with similar results.