Fig. 2.

Antiviral activity of PD and RW peptides during pre-infection, VV-binding and post-infection stage. PD and RW peptides were added to B-SC-1 cells at 3 different stages: (a) prior to infection (filled bars), (b) during virus binding (open bars) or (c) post-infection stage (shaded bars) and resulting viral titers were compared to that of VV-infection lacking the peptides. In the pre-infection experiment B-SC-1 cells were incubated with PD and RW peptides for 2 h at 37 °C, followed by washing of the cells and infection with VV. For the virus-binding stage experiment peptides were mixed individually with VV and the mixture was added to cells. Following incubation for 1 h inoculum was aspirated and agar overlay with DMEM was added. The post-infection assay was performed by first infecting B-SC-1 cells with VV for 1 h and then adding fresh medium containing PD and RW peptides. Note that both PD and RW peptides do not exhibit significant antiviral activity (p > 0.05) during these three stages of treatment.