Fig. 3.

Dose–response curves or EC₅₀ estimation of PD and RW peptides. Serial doubling dilution analysis was performed with PD3, PD4 and RW3 peptides. The final concentration of the PD peptides for the assay were 100, 50, 25 and 12.5 μg/ml whereas the RW peptides concentration was 10, 5, 2.5 and 1.25 μM. 1 ml of WR virus (10³ pfu/ml) was mixed individually with each of these peptides at RT for 2 h, followed by infection of B-SC-1 cells for 1 h. Following virus binding agar overlay with DMEM was added to cells and incubated at 37 °C for 72 h. Viral titers were estimated by counting plaques at the four different concentrations of the peptides tested and EC₅₀ values were deduced by using the GraphPad Prism 5.0 software. Peptide concentrations are represented on the x-axis (log scale) with PD3 and PD4 expressed in μg/ml and RW3 concentration expressed in μM. Analysis reveals that the EC₅₀ values for PD3 (A), PD4 (B) and RW3 (C) were 40 μg/ml, 50 μg/ml and 6.5 μM, respectively.