Fig. 1.

Screening for siRNA targeting NS3 gene. (A) Inhibition of CSFV replication by IFA in siRNA-treated cells (100× magnification). siC (siC transfection), NTC (mock transfection, stained with CSFV-negative serum), siNS3-1 (siNS3-1 transfection), siNS3-2 (siNS3-2 transfection), siNS3-3 (siNS3-3 transfection). (B) Reduction of viral genome replication in siRNA-treated cells. The CSFV genome copy numbers are the mean of three repeat experiments. (C) Inhibition of viable viral production in siRNA-treated cells. CCID₅₀ values are the means of three repeat titrations at the time points indicated. Ninety five percent confidence intervals are shown, ^∗P ⩽ 0.05, ^∗∗P ⩽ 0.01.