Table 1.
List of siRNA sequences in this study.
| Target namea | Sequences of siRNA (5′–3′)b |
|---|---|
| siN1 S | GACGGATCCGGATAGGTAGGGTGACAGGTTCAAGAGACCTGTCACCCTACCTATCCTTTTTTGGAAAAGCTTGCA |
| siN1 AS | TGCAAGCTTTTCCAAAAAAGGATAGGTAGGGTGACAGGTCTCTTGAACCTGTCACCCTACCTATCCGGATCCGTC |
| siN2 S | GACGGATCCGAACCCTGAAGTGGATTAGTTCAAGAGACTAATCCACTTCAGGGTTCTTTTTTGGAAAAGCTTGCA |
| siN2AS | TGCAAGCTTTTCCAAAAAAGAACCCTGAAGTGGATTAGTCTCTTGAACTAATCCACTTCAGGGTTCGGATCCGTC |
| siNS3-2 S | GACGGATCCGATGCCACAACCTAAGTTATTCAAGAGATAACTTAGGTTGTGGCATCTTTTTTGGAAAAGCTTGCA |
| siNS3-2 AS | TGCAAGCTTTTCCAAAAAAGATGCCACAACCTAAGTTATCTCTTGAATAACTTAGGTTGTGGCATCGGATCCGTC |
| siNS5B S | GACGGATCCGAATGAGAAGAGGGACGTCTTCAAGAGAGACGTCCCTCTTCTCATTCTTTTTTGGAAAAGCTTGCA |
| siNS5B AS | TGCAAGCTTTTCCAAAAAAGAATGAGAAGAGGGACGTCTCTCTTGAAGACGTCCCTCTTCTCATTCGGATCCGTC |
| siC S | GGAGGATCCGGACAGTGGGATGGATAGGTTCAAGAGACCTATCCATCCCACTGTCCTTTTTTGGAAAAGCTTCGG |
| siC AS | CCGAAGCTTTTCCAAAAAAGGACAGTGGGATGGATAGGTCTCTTGAACCTATCCATCCCACTGTCCGGATCCTCC |
a
S means sense strand, AS means antisense strand, C means control shRNA sequence.
b
For each target gene (sense strand as an example), complementary 75-mer oligonucleotides were designed with 5′ single-stranded overhangs (BamHI) and 3′ single-stranded overhangs (HindIII) for ligation into the pSilencer vectors. Sequences for enzyme sites are underlined. The oligonucleotides should encode 19-mer hairpin sequences specific to the mRNA target, a loop sequence separating the two complementary domains (TTCAAGAGA), and a polythymidine tract to terminate transcription (TTTTTT).