Table 2.
Primers used in this study.
| Primer name | Sequences (5′–3′) | Enzyme sites |
|---|---|---|
| H1-fw-n | CTTGCGGCCGCGGTTTTCCCAGTCACGAC | NotI |
| H1-rev-n | GCGGCGGCCGCGCACCCCAGGCTTTACACTTT | NotI |
| H1-fw-c | CTTATCGATGGTTTTCCCAGTCACGAC | ClaI |
| H1-rev-c | GCGATCGATGCACCCCAGGCTTTACACTTT | ClaI |
| Npro-fw | GGCCTCGAGATGGAGTTGAATCATTTTG | XhoI |
| Npro-rev | GCCCTGCAGGCAACTGGTAACCCACAATG | PstI |
| ΔNS3-fw | GGCCTCGAGATGAGAGTCTTGGTCTTGATCCC | XhoI |
| ΔNS3-rev | GCCCTGCAGTGCGGTCATGGCTACTACCCG | PstI |
| ΔNS5B-fw | GGCCTCGAGATGGGTGCTGCTGGTTTCTTTGAA | XhoI |
| ΔNS5B-rev | GCCCTGCAGCCATTCCCTCTTTACTTTGTC | PstI |
| S-Npro-fw | ATGGAGTTGAATCATTTTG | |
| S-Npro-rev | GCAACTGGTAACCCACAATG | |
| S-ΔNS3-fw | AGAGTCTTGGTCTTGATCCC | |
| S-ΔNS3-rev | TGCGGTCATGGCTACTACCCG | |
| S-ΔNS5B-fw | GGTGCTGCTGGTTTCTTTGAA | |
| S-ΔNS5B-rev | CCATTCCCTCTTTACTTTGTC |