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Inhibitory effects of emodin, rhein, and chrysin on the interaction between SARS-CoV S protein and ACE2. Biotin-labeled S protein (0.05 μg) was mixed with various amounts of emodin (A), rhein (B) or chrysin (C), and the mixtures were added to wells, which were coated with 1 ng of ACE2. The biotinylated ELISA was performed as described in Section 2. Results are expressed as inhibition (%) described in Section 2. Values are mean ± standard error of six independent assays.
